diff --git a/erpnext/stock/doctype/stock_entry/stock_entry.py b/erpnext/stock/doctype/stock_entry/stock_entry.py
index 48cfa51041..2f76bc7d56 100644
--- a/erpnext/stock/doctype/stock_entry/stock_entry.py
+++ b/erpnext/stock/doctype/stock_entry/stock_entry.py
@@ -76,6 +76,7 @@ class StockEntry(StockController):
 		self.validate_difference_account()
 		self.set_job_card_data()
 		self.set_purpose_for_stock_entry()
+		self.validate_duplicate_serial_no()
 
 		if not self.from_bom:
 			self.fg_completed_qty = 0.0
@@ -587,6 +588,22 @@ class StockEntry(StockController):
 			self.purpose = frappe.get_cached_value('Stock Entry Type',
 				self.stock_entry_type, 'purpose')
 
+	def validate_duplicate_serial_no(self):
+		warehouse_wise_serial_nos = {}
+
+		# In case of repack the source and target serial nos could be same
+		for warehouse in ['s_warehouse', 't_warehouse']:
+			serial_nos = []
+			for row in self.items:
+				if not (row.serial_no and row.get(warehouse)): continue
+
+				for sn in get_serial_nos(row.serial_no):
+					if sn in serial_nos:
+						frappe.throw(_('The serial no {0} has added multiple times in the stock entry {1}')
+							.format(frappe.bold(sn), self.name))
+
+					serial_nos.append(sn)
+
 	def validate_purchase_order(self):
 		"""Throw exception if more raw material is transferred against Purchase Order than in
 		the raw materials supplied table"""